ELISA kit experiments have no absorbance or low absorbance value how to break? - Database & Sql Blog Articles

During ELISA experiments, issues are common and inevitable. When problems arise, it's important to identify the root cause and then apply an appropriate solution based on that reason. In this article, we will discuss a specific issue: what to do when there is no absorbance or low absorbance after adding the reaction stop solution in an ELISA experiment.

After adding the reaction stop solution, if there is no absorbance or the absorbance is very low, here are some possible causes and solutions:

a. Cause: The enzyme-labeled reagent was not added at all.

Solution: Make sure you follow the protocol step by step and double-check that all required components have been included.

b. Cause: The kit components were not fully reconstituted or mixed properly.

Solution: Ensure that all reagents are brought to room temperature and thoroughly mixed before use.

c. Cause: The ambient temperature is too low during incubation.

Solution: If the room temperature is below 19°C, consider extending the incubation time slightly in step 4 to allow for proper reaction development.

d. Cause: The washing steps were skipped or not performed with the correct wash buffer from the kit.

Solution: Always use the designated washing solution provided in the kit to ensure accurate results and reduce background noise.

e. Cause: The ELISA kit has expired or is past its shelf life.

Solution: Replace the kit with a new one that has a valid expiration date to avoid unreliable results.

If you encounter any technical difficulties during your ELISA experiments, don't hesitate to reach out for expert support. Shanghai Jinma offers one-on-one technical guidance throughout the entire process, ensuring you get reliable results every time. We provide high-quality ELISA test kits, including human, rat, and mouse ELISA kits, along with biological reagents, sera, antibodies, media, standards, and domestic serum. We also offer GIBCO fetal bovine serum for researchers who require premium quality materials.

Whether you're a beginner or an experienced researcher, our team is here to help you troubleshoot and succeed in your experiments. Don’t let unexpected issues slow you down—contact us today for personalized assistance and top-notch products!